PittsburghV1, Main, Exploration, bibRecord, 000A17

Understanding dynamic changes in live cell adhesion with neutron reflectometry.

Identifieur interne : 000A17 ( Main/Exploration ); précédent : 000A16; suivant : 000A18

Understanding dynamic changes in live cell adhesion with neutron reflectometry.

Auteurs : Ann Junghans [États-Unis] ; Mary Jo Waltman [États-Unis] ; Hillary L. Smith [États-Unis] ; Luka Pocivavsek [États-Unis] ; Noureddine Zebda [Royaume-Uni] ; Konstantin Birukov [États-Unis] ; Mariano Viapiano [États-Unis] ; Jaroslaw Majewski [États-Unis]

Source :

Modern physics letters. B, Condensed matter physics, statistical physics, applied physics [ 0217-9849 ] ; 2014.

RBID : pubmed:25705067

Abstract

Neutron reflectometry (NR) was used to examine various live cells adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell - surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.

DOI: 10.1142/S0217984914300154
PubMed: 25705067

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Neutron reflectometry (NR) was used to examine various live cells adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell - surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.</div>
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Understanding dynamic changes in live cell adhesion with neutron reflectometry.

Understanding dynamic changes in live cell adhesion with neutron reflectometry.

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